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KMID : 1005220080230030211
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Journal of Embryo Transfer
2008 Volume.23 No. 3 p.211 ~ p.216
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Identification of Inflammation-related Genes Altered in the Cystic Ovary and Endometritis of Korean Cattle
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Choe Chang-Yong
Park Sun-Young
Kim Eun-Sook
Moon Yoon-Ja
Park Hye-Jin
Son Dong-Soo
Cho Sang-Rae
Kim Hyun-Jong
Kim Jae-Bum
Park Jae-Yong
Hong Seong-Geun
Han Jae-Hee
Kang Da-Won
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Abstract
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This study was carried out to investigate inflammation-related gene expression altered in ovary and endometrium of Korean cattle with reproductive disorders using microarray. In the present study, nine inflammation-related differential1y expressed genes (DEGs) were identified in the cystic ovary and endometrium with endometritis. In the follicular cyst, eotaxin and alpha-2-HS-glycoprotein (AHSG) were up-regulated, whereas complement component 3 (C3) and oxidised low density lipoprotein (lectin-like) receptor 1 (OLR1) were down-regulated. Complement component 4A (C4A) was up-regulated in luteal cyst. In the endometritis, chemokine 1igand l and 2 (CXCL1 and CXCL2), protein C (inactivator of coagulation factors Va and VIIIa), and complement component C5 were up-regulated, whereas kininogen was down-regulated. Of these genes, we focused on eotaxin and kininogen, which were highly regulated in the follicular cyst and endometritis, respectively and on C3 commonly regulated in both reproductive disorders. The microarray data of eotaxin, kininogen, and C3 were validated by semi-quantitative PCR. Consistent with microarray data, eotaxin was up-regulated by 4-fold in the follicular cyst, while kininogen was down-regulated by 5-fold in the endometritis. C3 was down-regulated in the both follicular cyst and endometritis. Our results suggest that these inflammation-related genes could be useful markers for diagnosis of cystic ovary and endometritis of Korean cattle.
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KEYWORD
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Korean cattle, microarray, follicular cyst, endometritis
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